Abstract
ABSTRACTLysozyme plays a central role in initiating and maintaining the antibacterial defense response of insect. A new family member of insect lysozyme, an antibacterial peptide, has been isolated from the hemolymph of the fifth instar larvae ofAgrius convolvuli. Vaccination was proceeded withE. coliK12 D21 (4x106cells of log phase) injection into the abdomen of the larvae.Agriuslysozyme was isolated by cation‐exchange chromatography and RP‐FPLC, and sequenced by HPLC system. It was observed that the purifiedAgriuslysozyme was heat‐stable and had a molecular weight of approximately 15 kDa by SDS‐PAGE. The N‐terminal amino acid sequence ofAgriuslysozyme is K‐H‐F‐S‐R‐C‐G‐L‐V‐Q‐E‐L‐F‐W‐Q‐G‐F‐P with the highest similarity to that ofHeliothis virescence, and it has been identified that theAgriuslysozyme belongs toctype lysozyme.
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