Structure determination of O-linked carbohydrate moiety of HemaP-like peptide, a novel feeding-modulating peptide from the sweet potato hornworm, Agrius convolvuli

Abstract

Event Abstract Back to Event Structure determination of O-linked carbohydrate moiety of HemaP-like peptide, a novel feeding-modulating peptide from the sweet potato hornworm, Agrius convolvuli Nobukatsu Morooka1*, Junpei Shinji1, Koji Shirai2, Kenji Kiguchi2 and Hiromichi Nagasawa1 1 The University of Tokyo, Graduate School of Agricultural and Life Sciences,, Japan 2 Shinshu University, Division of Applied Biology, Japan We have recently identified a novel feeding-modulating peptide, HemaP (hemolymph major anionic peptide) from the silkworm, Bombyx mori (in preparation). HemaP consists of 62 amino acid residues, and is predominantly produced by the fat body. Injection of HemaP into Bombyx larvae induces foraging behaviors such as nibbling and ingesting. Datebase search indicated that HemaP is an unknown peptidyl factor and is conserved among lepidopteran insects. To characterize the HemaP-like peptide from other lepidopteran insects, we tried to purify it from larval hemolymph of Agrius convolvuli, taxonomically closely related to the tobacco hornworm, Manduca sexta. Finally, we isolated two distinct HemaP-like peptides and designated those two peptides p6692 and p6894 according to their molecular masses. N-terminal amino acid sequence and ESI MS/MS analyses of p6692 and p6894 revealed that they had the same amino acid sequence but were different in mass by 202, corresponding to that of N-acetylhexosamine and that the N-acetylhexosamine was linked to Thr5. To determine the structure of the carbohydrate moiety, p6894 was hydrolysed in TFA, followed by labeling with 2-aminobenzoic acid (2AA). The resulting 2AA derivative was identified as 2AA-GalNAc by the elution time on reversed-phase HPLC. Since p6894 was recognized by GSLI, a lectin, specific for a-Gal and a-GalNAc, we concluded that p6894 linked a-GalNAc. Although Agrius hemolymph had two isoforms of HemaP-like peptides with or without a carbohydrate moiety, which was not the case in Bombyx HemaP, they both showed almost comparable activity of inducing foraging behaviors in Agrius larvae. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Morooka N, Shinji J, Shirai K, Kiguchi K and Nagasawa H (2010). Structure determination of O-linked carbohydrate moiety of HemaP-like peptide, a novel feeding-modulating peptide from the sweet potato hornworm, Agrius convolvuli. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00052 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Mr. Nobukatsu Morooka, The University of Tokyo, Graduate School of Agricultural and Life Sciences,, Tokyo, Japan, aa087021@mail.ecc.u-tokyo.ac.jp Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nobukatsu Morooka Junpei Shinji Koji Shirai Kenji Kiguchi Hiromichi Nagasawa Google Nobukatsu Morooka Junpei Shinji Koji Shirai Kenji Kiguchi Hiromichi Nagasawa Google Scholar Nobukatsu Morooka Junpei Shinji Koji Shirai Kenji Kiguchi Hiromichi Nagasawa PubMed Nobukatsu Morooka Junpei Shinji Koji Shirai Kenji Kiguchi Hiromichi Nagasawa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.