Abstract
Balb/C mice were immunized with crude Portuguese Man-O'War ( Physalia physalis) nematocyst venom and their spleen immunocytes were fused with plasmacytoma cells. Nine hybridomas which produced IgG specific for Man-O'War venom were identified using a specific ELISA technique. Ammonium sulfate and DEAE cellulose-purified monoclonal anti-venom antibody had an ELISA titer of 1 :4000 and an ability to neutralize the lethal activity (4 ld 50/0.6 ml ascites fluid) of an i.v. challenge of crude venom. Indirect immunofluorescence testing demonstrated that the monoclonal antibody isolated in these experiments reacted against a venom component located in the nematocyst wall and thread.
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