Isolation of Gamma Ray Induced Urd Bean [Vigna mungo (L.) hepper] Mutants with Improved Batter Quality

Abstract

Background: The urd bean variety MDU 1 has a duration of 70-75 days, bushy in nature and is susceptible to yellow mosaic virus. In order to develop a determinate type and improved batter quality, MDU 1 and VBN (Bg) 4 blackgram seeds were treated with different doses (100 Gy, 200 Gy, 300 Gy, 400 Gy and 500 Gy) of gamma rays. Methods: Uniform sized seeds treated with different mutagenic doses were raised in randomised block design which constitute M1 generation. Each plant was harvested individually and forwarded to M2 generation following plant to progeny row method. The determinate types were selected from M2 generation and forwarded to further generations. After attaining homozygosity in the advanced generation, the mutants were checked for its biochemical characters (Total solubule protein content analysed by kjeldahl method, albumin and globulin analysed by Lowery’s method, arabinose content analysed by Bial method. Result: The mutants ACM - 16 -011, ACM - 16 -015, ACM - 16 -018 were found to have an arabinose content of 8.28%, 8.98% and 8.14% respectively. All these mutants recorded more batter volume over the variety MDU 1. The albumin (%) and globulin (%) contents were also found at remarkably increased levels in the mutants. These mutants have the potential to develop a high quality variety of urd bean and therefore are very useful in breeding programme.