Abstract
Knowledge of the microbiology of dinitrogen (N 2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N 2)-fixing bacteria from DPS composts were isolated and studied for their N 2-fixing activity in vitro and in vivo. Two Gram-negative N 2-fixing isolates were identified as Pseudomonas. At 20 °C, both isolates revealed that N 2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N 2-fixing activity was found to occur between 18 and 25 °C, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N 2-fixing activity after carbohydrate amendments both with and without inoculation of a N 2-fixing isolate. These results suggest that DPS composts support N 2-fixing bacteria and that N 2-fixing activity is dependent on a usable carbohydrate source.
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