Isolation of ferric Yersinia bactin A (fyuA) as virulence gene and biofilm forming in Escherichia coli was Collected from patient with UTI.

Abstract

The focus of the current investigation was applied on determining the presence of ferric yersiniabactin uptake A (fyuA) virulence gene and identifying the antibiotic resistance properties in biofilm forming Escherichia coli­­ isolated from recurrent urinary tract infection patients and healthy individuals. In the Microbiology laboratory at Al Yarmouk hospital in Baghdad, Iraq, 20 (16 females and 4 males) UTI-diagnosed patient urine and stool samples, as well as 20 (10 females and 10 males) healthy individual stool samples, were collected. The samples were exposure to series test including O and H antigen based identification using an antibody (polyvalent and monovalent)-directed agglutination test, antibiotic sensitivity testing using the Kirby Bauer disc diffusion method, Congo-red agar method-based biofilm formation identification, and virulence gene identification using polymerase chain reaction (PCR).Results: 40 and 20 strains were discovered from UTI patients and healthy persons, respectively, in which O serogroups were found in 7(35%) and 3(15%) of those strains, respectively. Furthermore, only two strains of enterohemorrhagic E. coli (O157 H7) and enteroaggregative E. coli (O44 H7) were found in the UTI-based urine samples. Multidrug resistance (MDR) was detected in 60% of the biofilm positive producers (BPPs) isolated from UTI urine samples; however, only 20% of the biofilm negative producers (BNPs) isolated from UTI urine samples displayed MDR features. In the instance of virulence genes, a link was shown between fyuA (a gene that codes for an outer-membrane protein called ferric yersiniabactin uptake) and biofilm in 75% and 60% of UTI urine and stool strains, respectively, and in 55% of healthy individual stool strains. The findings of the antibiotic sensitivity test revealed that the UTI strains were 90 percent, 100 percent, 65 percent, and 40 percent sensitive to imipenem, amikacin, gentamicin, and tobramycin, respectively. Conclusion:The present research offers detailed information on the biofilm, virulence gene, and antibiotic resistance features of Escherichia coli from recurrent urinary tract infection patients and healthy people.