Isolation of Bacteria From Sub-Clinical Cases in Mastitic She-Camel (Camelus Dromedaries) and Their Sensitivity to Some Antibiotics

Abstract

The objective of this study was to determine the bacteria associated with mastitis in she- camel and to evaluate the sensitivity of these bacteria to antibiotics. Three hundred and thirty seven milk samples were collected from she- camel from Red Sea state and examined by WST, 40(11.87%) were positive and 297(88.13%) were negative. From the positive samples 115 organism were isolated. Isolation and identification of the bacteria is according to a standard cultures technique. It resulted in that the most predominant cause of she-camel mastitis is Staphylococcus spp. (74%). The other causes were Streptococcus spp.(15%), Corynebacterium spp.(1%), Anaerobic cocci spp.(2%), Bacillus spp.(4%),and Micrococcus spp.(4%).The Staphylococcus spp. isolated were S.aureus (58.07%), S.epidermidis(1.74%),S.hyicus (2.61%),S.simulans (0.87%), S. warnei (1.74%), S.lominis (0.87%), S.kloosii (5.22%), S.sciuri (0.27%) and S.lentus (2.61%) The sensitivity of these bacterial isolates to antibiotics was done using Oxoid discs of Ampicillin, Erthomycin, kanamycin, Streotomycin, tetracycline, chloramphenicol, Gentamycin and nalidixic acid. The organisms were considered sensitive or resistant according to zone size.The bacteriostatic and bactericidal activity of each drug was done. The results of the sensitivity test showed that the Staphylococcus spp. were highly sensitive to Ampicillin, Tetracycline and Gentamycin, while they are resistant to Erythromycin and Nalidixic acid. The Streptococcus aglactiae, Strep.dysglactiae and Strep uberis were sensitive to all tested antibiotic preparations, but resistant to Nalidixic acid antibiotic. The sensitivity of other isolates was not significant compared to the major isolates. It is concluded that the most dominant cause of mastitis in she- camels of the Red-Sea State of Sudan is Staphylococcus spp. and the most effective antibiotics against most of the isolated organisms are Tetracycline, chloramphenicol Gentamycin, Ampicillin and kanamycin.