Isolation of annonacous acetogenins from Annona muricata fruit and their anti proliferative activity on human prostate cancer cell PC-3

Abstract

The fruit of Annona muricata is an edible and medicinal tropical fruit and commonly available in local markets. The previous work was continued, bioassay-guided fractionation of the fruit powder of A. muricata yielded three more novel bioactive compounds: C-35 annonaceous acetogenins, muricins M and N, and C-37 annonaceous acetogenins, muricenin. They all contain a mono-tetrahydrofuran ring and four hydroxyl groups. The structures were elucidated by spectral methods and chemical modification after isolation via open column chromatographic separation and HPLC purification. Especially, murices M and N demonstrated more potent anti-proliferative activities against human prostate cancer PC-3 cells.