Abstract
This report documents the first isolation of Acholeplasma laidlawii from a commercial lot of serum-free Dulbecco basal medium. Experimental studies demonstrated survival of the organism for at least 1 year depending on the concentration of the contaminating organism as well as pH and temperature of storage of the serum-free medium. A comparison of isolation methods showed that concentration by filtration through 220-nm membrane filters and testing the filters for mycoplasma recovery, especially when using phenol red-diphasic Hayflick medium, was both sensitive and practical for the average laboratory.
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