Abstract
Microsatellite primers pairs were developed for the Neotropical tree Roupala montana var. brasiliensis for use in studies on genetic diversity, mating system, and gene flow. Forty-two primer pairs were developed, resulting in 27 polymorphic loci, with two to 27 alleles per locus. The primer pairs were validated against 34 R. montana var. brasiliensis adult trees from four populations. The observed (H o) and expected (H e)heterozygosities ranged among loci from 0.061 to 0.930 (mean of 0.544) and from 0.116 to 0.950 (mean of 0.700), respectively. Null alleles were observed for ten loci. No genotypic linkage disequilibrium was detected in any pair of loci. This set of loci is suitable for population genetic studies of the species.
Highlights
Twenty-one species and variations of the Roupala (Proteaceae) genus have been reported in Brazil (Prance et al 2007), including lacewood (Roupala montana var. brasiliensis (Klotzsch) K.S
We describe the isolation of 27 nuclear microsatellite markers for R. montana var. brasiliensis that provide the foundation for further research on genetic diversity, population structure, mating system, conservation genetics, and possibly assist breeding programs
Total genomic DNA was extracted from leaves of a single R. montana var. brasiliensis individual (Supplementary Material - Table SI, using the protocol based on Doyle & Doyle (1990) proposed by Faleiro et al (2003), with modifications
Summary
Twenty-one species and variations of the Roupala (Proteaceae) genus have been reported in Brazil (Prance et al 2007), including lacewood (Roupala montana var. brasiliensis (Klotzsch) K.S. Microsatellite loci or Simple Sequence Repeats (SSR) are highly informative molecular markers because of the differences in the number of repeated units; they are The limiting factor in the use of microsatellites is obtaining primers to amplify the SSR loci.
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