Abstract
The study was carried out from November, 2014 to February, 2015 with the objective of isolating Aeromonas hydrophila an important fish pathogen from infected air-breathing catfish Magur (Clarias batrachus) in Mymensingh district. Quantitative study of the isolated bacteria from infected C. batrachus was found variation of number in different organs. Total bacterial load was found to be 1.16 x 105 to 3.15 x 106 cfu/g in lesions, 2.14 x 108 to 4.17 x 109 cfu/g in liver, 1.90 x 107 to 5.12 x 108 cfu/g in spleen and 2.32 x 106 to 5.24 x 108 cfu/g in kidney of infected C. batrachus in Mymensingh district. The isolates were found to produce acid from arabinose, whereas acid and gas from different sugar media such as maltose, sucrose, and dextrose. Morover, they were capable to ferment glucose but resistant to vibriostatic agent 0129 test. Further identification of A. hydrophila was accomplished using PCR. The PCR products of desired 760 bp were obtained for A. hydrophila. The isolated A. hydrophila were 96% sensitive to Enrofloxacin followed by 88% to Ciprofloxacin and 76% to Levofloxacin. On the other way, 100% were resistant to the Ampicillin followed by 96% to Penicillin and 92% to Novobiocin. So far, this is the first molecular identification of A. hydrophila from farmed C. batrachus in Bangladesh. The present study will provide future research scopes on identification of pathogenicity island in chromosome and serotyping of all A. hydrophila isolates.
 Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 17-24
Highlights
Among the different air-breathing catfishes, Magur (Clarias batrachus) is very popular and highly valuable fish species in Bangladesh
The appropriate breeding, nursing and rearing technology of fry and fingerlings of C. batrachus had been developed by Bangladesh Fisheries Research Institute (BFRI) in few years ago but unknown diseases of C. batrachus causes serious economic losses because of their high mortality
Most of the diseased fish were collected in a live condition and the samples were transported in well-aerated water to the Fish Disease and Health Management Laboratory of Bangladesh Fisheries Research Institute (BFRI), Mymensingh
Summary
Among the different air-breathing catfishes, Magur (Clarias batrachus) is very popular and highly valuable fish species in Bangladesh. It is recognized for its delicious taste and market value but it is considered as a medicinal fish and traditionally remained a strike among the pregnant & lactating mothers, the elderly and children. Most of the catfishes are very hardy that can survive for quite a few hours outside the water due to presence of accessory respiratory organs (Khan et al, 2003; Monir and Rahman, 2015). C. batrachus was abundantly available in open water of Bangladesh but presently, it is threatened due to over exploitation and various ecological changes in its natural habitat.
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