Abstract
Fish play a crucial role in the Bangladeshi diet, providing more than 60% of animal source food, representing a crucial source of micro-nutrients and possessing an extremely strong cultural attachment. In this study isolation and identification of Aeromionas hydrophila was done by studying cultural properties, Grams staining and biochemical properties of isolates of diseased indigenous Koi fish (Anabas testudineus) of different upazillas of Mymensingh district. Antibiogram profile of the isolated bacteria was studied by using wide range of commercially available antibiotics. Quantitative study of bacteria isolated from diseased indigenous Koi fish showed variation of number in different organ. Total bacterial load was found to be 1.90 × 105, 1.19 × 105, 3.21 × 105, 2.18 × 106 and 3.14 × 105 cfu/g in lesions; 2.52 × 107, 2.34 × 108, 5.41 × 108, 2.54 × 109 and 5.21 × 109 cfu/g in liver; 2.54 × 108, 2.41 × 108, 1.90 × 107, 3.65 × 107 and 3.45 × 108 cfu/g in spleen; 3.51 × 107, 5.28 × 107, 3.14 × 106, 1.85 × 107 and 4.52× 107 cfu/g in kidney in diseased Koi of Mymensingh sadar, Muktagacha, Tarakanda, Gouripur and Fulpur upazillas, respectively under Mymensingh districts. Aeromonas hydrophila was initially identified by their specific morphological, physiological and biochemical characteristics. Then molecular detection of A. hydrophila was done by PCR. PCR products of desired 760 bp were obtained for A. hydrophila. The results of the antibiotic sensitivity test is exhibited that most of the bacterial samples were sensitive against ciprofloxacin (92%) and levofloxacin (84%), intermediate resistant against gentamicin (40%) and resistant against novobiocin (84%), ampicillin (100%) and penicillin (92%).Asian J. Med. Biol. Res. June 2016, 2(2): 332-340
Highlights
The Climbing Perch fish Anabas testudineus (Bloch, 1792) is one of the important small indigenous species (SIS), fresh water fish of Bangladesh, which is locally known as Koi in different places of Bangladesh
The objectives of the present study were isolation, identification and molecular detection of actual disease causing agent which is responsible for mass mortality of cultured indigenous Koi (A. testudineus) of Mymensingh district of Bangladesh
Selection of fish farms and study area Different Climbing Perch, Koi (A. testudineus) farms of Muktagacha, Tarakanda, Gouripur, Fulpur, Sadar upazillas under Mymensingh district located at 24o38 3 N 90o16 4 E of Bangladesh were selected to collect infected Koi fish samples for isolation, identification and molecular detection of actual pethogenic agent and evaluate their antimicrobial resistance patterns
Summary
The Climbing Perch fish Anabas testudineus (Bloch, 1792) is one of the important small indigenous species (SIS), fresh water fish of Bangladesh, which is locally known as Koi in different places of Bangladesh. This fish is native in Southeast Asian region, often found in fresh water sources of east India and south China (Chakraborty et al, 2014). It is commonly found in open water (streams, lakes, floodplain and beels), paddy fields and swamps of Bangladesh and its preferred habitats are heavily-vegetated, stagnant waters. According to Saha et al, 1971, the fish contain high values of physiologically available iron and copper essentially needed for hemoglobin synthesis This fish was abundantly available in our open water system but due to over exploitation and various ecological changes in its natural habitat; this native species is declining. Most pond fish farmers of Climbing Perch do not have a good understanding of health and disease issues in their system (Hasan et al, 2013). The objectives of the present study were isolation, identification and molecular detection of actual disease causing agent which is responsible for mass mortality of cultured indigenous Koi (A. testudineus) of Mymensingh district of Bangladesh
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