Isolation, identification, antibiotic susceptibilities and SDS-PAGE protein profiles of Staphylococcus aureus strains obtained from She- camel milk eastern Libya

Abstract

Staphylococcus aureus (S. aureus) is instinctively present in milk and dairy products and associated with many outbreaks. Milk is a good substrate for S. aureus rise and toxin production. When transmitted to human body this pathogen may affect skin, brain, kidney, liver and several other vital organs. Therefore, the pathological effects of S. aureus have been the focus of several recent research works. The objective of this study was to evaluate Antibiotic Susceptibilities of S. aureus strains and to Identify their Protein Profiles using SDS-PAGE. S. aureus were isolated from different camel milk sample in Libya and identified using microbiological and molecular techniques. The AVIPATH-STAPH test was performed for detection of coagulase and Protein A, both factors are associated with S. aureus. All S. aureus strains were tested for susceptibility to a panel of 9 antimicrobial disks by the disc–agar diffusion method on Mueller -Hinton agar (oxoid), following the National Committee on Clinical Laboratory Standard. Total Soluble Protein Profiles of S. aureus strains were analyzed by SDS-PAGE. Results of this study showed that, all S. aureus strains were resistance to the 9 antibiotics tested. Regarding to protein profiles results of S. aureus strains, it seems to be different from each other as in Polymorphism Analysis, protein bands and dendrogram analysis. This study confirmed the potential risk of consuming raw she-camel milk, especially upon lack of strict hygienic and preventative measures to avoid the presence of S. aureus in milk.