Isolation, Identification And Toxin Production From Vaginal Clinical Isolates Of Candida Albicans

Abstract

Thirty two isolates of candida albican were sequestered from female patients with vaginal ulcer (lying in marjan hospital hillia city) .The vaginal swap were cultured on  sabouraud dextrose broth and agar plate respectively . for differential identification of C. albican, hi chrome candida agar was used. Gram staining used to smear of vaginal scrapings. suspected isolates of C.albican then immunized on corn meal agar  mediocre for chlamydospore, formation , for germ tube formation the isolates of  Caliban was cultured in human serum .Carbohydrate assimilation and fe mentation  tests also used for further identification . four identification s trains of C.albican namely (  CA1, CA2, CA3, CA4) were selected according to their antagonistic activity against  standard strain of bacteria E.coli NCTC5933 and staphylococcus aureus NCTC6571 ;  To produced extracellular toxin of glycoprotein nature in solid media and fermentation  liquid media . The toxin which produced (secondary metabolites) don’t effecting the producers strains of C.albican (CA1,CA2,CA3,CA4) ,but the produced toxin was  inhibited the growth of standard strain of candida albicans ATcc8201( sensitive ).  Simple method used for toxin extraction by using ethanol 90%. And the extracted toxin  from free cells supernatant . cytotoxicity of toxin against human blood cells were 6 ppm also, the stability of producing toxin was determined.