Isolation, Identification, and Process Optimization of Novel Strain Bacillus paramycoides from Food Waste

Abstract

A new strain of alkaline protease-producing bacteria was isolated from soil and identified via 16s rRNA sequencing. The bacteria was identified as a novel strain of Bacillus paramycoides. Optimized substrate concentrations were used while varying one factor at a time to determine optimal process conditions. The bacterium isolated from slaughterhouse soil was found to show maximum enzymatic activity. Among the various substrates, potato peel (3%) and mustard oil cake (2%) were shown to be effective carbon and nitrogen sources, respectively. Alkaline protease production activities for potato peel and mustard oil cake were determined to be 1436.7 U/mL and 1425 U/mL, respectively. Maximum enzymatic activity (2752.5 U/mL) was observed at pH 9, temperature 40°C, inoculum age 12 h, inoculum size 2%, and production time 72 h. The low cost of enzyme with high activity at mild conditions suggests its potential use in the food industry. To the best of our knowledge, this is the first study on isolation, identification and process optimization of novel strain B. paramycoides from food waste.