ISOLATION, IDENTIFICATION, AND MEDIA OPTIMIZATION OF HIGH-LEVEL CELLULASE PRODUCTION BY Bacillus sp. BCCS A3, IN A FERMENTATION SYSTEM USING RESPONSE SURFACE METHODOLOGY

Abstract

Cellulases are important glycosyl hydrolase enzymes, which break down cellulose to β-glucose. They have been used widely in biotechnological processing such as bioethanol production. In this work we studied maximizing cellulase production by Bacillus sp. BCCS A3 using response surface methodology (RSM). A good result was attained with these conditions (% w/v): tryptone 0.1, Na2PO4 0.25, (NH4)2SO4 0.2, MgSO4 · 7H2O 0.005, CaCl2 0.005, KH2PO4 0.1, NaCl 0.1, sodium carboxymethylcellulose (CMC) 0.75, and pH 9. The cellulase activity in optimized medium was 49.80 U/ml. Moreover, high level of enzyme production was obtained by using fermentor system (50.30 U/ml). Thus, according to the obtained results, this statistical method provided quick identification and integration of key medium details for Bacillus sp. BCCS A3, leading to more cellulase production.