Abstract
In the present study, out of 255 milk samples (185 from subclinical and 70 from clinical cases) screened for Staphylococcus aureus, 53 isolates were obtained giving an overall incidence of 20.78%. The incidence of S. aureus from subclinical mastitis was 8.11% (15/185) and from clinical mastitis was 54.29% (38/70). Species-wise incidence was 30.77% (16/52) in buffaloes and 18.23% (37/203) in cows. All 53 isolates were identified on the basis of colony characters on nutrient agar, staining and biochemical characters namely catalase production, oxidise test, maltose fermentation test and ohosphatase test. Out of 53 isolates, 49 (92.45%) isolates showed coagulase production in tube coagulase test. Number of isolates showing alpha, beta, gamma and alpha-beta haemolysin production were 18 (33.96%), 26 (49.06%), 4 (7.55%) and 5 (9.43%) on sheep blood agar, respectively. All the 53 S. aureus isolates were confirmed genotypically by 23S rRNA ribotyping in which a species-specific amplicon of 1, 250 bp was obtained. Antibiotic sensitivity patterns of all these 53 S. aureus isolates revealed that the isolates were most sensitive to gentamicin (94.34%), whereas penicillin (94.34%) and ampicillin (86.79%) were highly resistant to S. aureus.
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