Abstract
Hydropericardium syndrome is an important emerging disease of domestic fowl caused by fowl adenovirus serotype 4 (FAV-4). The full length hexon gene was isolated by PCR from the three virus isolates and it has been possible to clone hexon gene from all the three isolates into pGEM-T Easy vector, sequenced and analyzed using online bioinformatics tools (ExPASy server). The sequences were submitted to NCBI Gen Bank. These sequences showed significant homology with FAV-4 hexon gene sequences available in the NCBI Gen Bank and also found to be clustered with FAV-9 and FAV-10 serotypes. Probable secondary structures were also studied and compared using on line ExPASy servers. Possible B-cell epitopes were also predicted using on line ABCpred server and their position on the 3D structure of the proteins were also studied.
Highlights
Poultry keeping in India has emerged as one of the fastest growing sector of Indian agriculture
Consequent to intensification, many nonexistent diseases have emerged and some of the existing has appeared in altered form, viz., very virulent infectious bursal disease, hydropericardium syndrome (HPS), chicken infectious anaemia (CIA), egg-drop syndrome 76 (EDS-76), reoviral infections and infectious bronchitis
Hydropericardium syndrome (HPS) is caused by fowl adenovirus 4 (FAV-4), which belongs to genus Aviadenovirus of family Adenoviridae [14]
Summary
Poultry keeping in India has emerged as one of the fastest growing sector of Indian agriculture. HPS is an important emerging disease occurring in specific areas of world where broilers are reared under intensive conditions. The identification and characterization of structural proteins of FAV-4 prevalent in a geographical area is a necessary prerequisite to investigate possible variation in polypeptide composition and to understand their immunogenic property, which may in turn help in the development of suitable immunodiagnostic and immunoprophylaxis. Hexon, being the major immunogenic protein and harbouring the type, group and sub-group specific determinants, is of prime importance in molecular epidemiology of the virus. The present study was performed to characterize the hexon gene from Indian isolates of FAV-4 after PCR amplification, cloning of the amplicons into the suitable vectors followed by phylogenetic analysis, primary and secondary structure analysis of the hexon protein
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