Abstract
The genus Neosartorya, the sexual states of Aspergillus, belongs to the Eurotiales which have been reported for compounds with bioactivities such as antimicrobial, antimalarial and cytotoxicity. The objective of this research was to isolate, identify and screening for anticancer and antimicrobial properties of the ethyl acetate crude extract from Neosartorya. Neosartorya sp. was isolated from soil in Chiang Mai province, Thailand by the soil plate technique. The samples were identified base on morphology and DNA sequencing: Internal transcribed spacer (ITS), s-tubulin and calmodulin as Neosartorya hiratsukae and Neosartorya pseudofischeri. For in vitro cytotoxicity activity by MTT assay using eight cell lines (MCF-7, P388, HeLa, Vero, L929, KB, HT-29 and HepG2). The crude ethyl acetate extract of N. hiratsukae and N. pseudofischeri showed the highest potency against the L929 cell line with the 50% inhibitory concentration (IC50) value of 144.31 and 267.73µg/ml, respectively. The antimicrobial properties of crude extract were evaluated using disc diffusion method against five bacteria strain: Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli and Pseudomonas aeruginosa. The extract was well inhibited the growth of gram positive pathogenic bacteria: B. subtilis, S. aureus and M. luteus. The results indicate its possible potential with the production of other bioactive agents for future anticancer and antibiotic drug development.
Highlights
The genus Neosartorya belongs to Trichocomaceae family under the order Eurotiales
Three new compounds isolated from the Neosartorya pseudofischeri inhibited the growth of six human cancer cell lines that a quinazolinone displayed cytostatic effects in human U373 glioblastoma and A549 non-small cell lung cancer apoptosis-resistant cells with marked inhibition of mitotic rates [1]
The morphological features were observed as microscopic characters which showed white to creamy colony and cleistothecia, ascospores are globose to broadly ellipsoidal with equatorial furrow and ridges identified as Neosartorya
Summary
The genus Neosartorya belongs to Trichocomaceae family under the order Eurotiales. These fungi are perfect state (teleomorphic stage) of Aspergillus. Some species of the genus Neosartorya have been reported for compounds with bioactivities such as antibacterial and cytotoxicity. Three new compounds isolated from the Neosartorya pseudofischeri inhibited the growth of six human cancer cell lines that a quinazolinone displayed cytostatic effects in human U373 glioblastoma and A549 non-small cell lung cancer apoptosis-resistant cells with marked inhibition of mitotic rates [1]. A new meroterpenoid: tatenoic acid was isolated from the fungus N. tatenoi exhibited cytotoxicity against human small cell lung cancer (NCI-H187) and human epidermoid carcinoma in the mouth (KB) [3]. This research focuses on isolation and identification of Neosartorya from the forest soil in Thailand and screening for anticancer and antimicrobial properties
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