Isolation Bac. anthracis at different pH and soil humus content

Abstract

Anthrax is one of the diseases that cause great damage to livestock and at the same time is a dangerous disease for humans. This disease under different names is known from ancient times. The source of the pathogen is diseased animals. However, an important factor in the transmission of the pathogen is the burial place of animals that died from anthrax. The pathogen can be stored in the soil for more than 100 years. Anthrax is one of the diseases that cause great damage to livestock and at the same time is a dangerous disease for humans. This disease under different names is known from ancient times. The source of the pathogen is diseased animals. However, an important factor in the transmission of the pathogen is the burial place of animals that died from anthrax. In Ukraine there is burial of dead animals from anthrax. Most of the Siberian burial ground is registered in Vinnytsia, Kharkiv, Chernihiv, Ternopil, Lugansk and Kirovograd regions of Ukraine. In this regard, there is a need to study the issues of epizootology and improve diagnostic techniques, measures for general and specific anthrax prevention. The pathogen can be stored in the soil for more than 100 years. The purpose of these studies was to select the Bacillus anthracis spores for the influence of pH and different humus content in the soil. The pH and soil humus content were determined, which was used in experiments at the Laboratory of Agrochemistry of the Institute of Bioenergetic Cultures and Sugar Beet at the National Academy of Agrarian Sciences of Ukraine. The work was performed on the basis of the State Scientific-Control Institute of Biotechnology and strains of microorganisms. Used methodology in which soil sample – 2.5 grams grow. In studies, spores of the avirulence strain Bacillus anthracis were introduced into a sterile soil. Registered methods of separating the argument of anthracite from the soil are obsolete. The method allows distinguishing anthrax spores from soils with different pH and humus content. when 21.76 ± 0.94 spores were applied to sterile soil (which was 17.41 ± 0.75 s/gram), the amount of spores selected was 3.33 ± 0.27 spores/grams (19.13%). The amount of controversy in the experimental soil was lower (17.41 ± 0.75), compared with the amount of controversy submitted to the soil with a pH of 6.5 (P < 0.05), 6.7 (P < 0.05), 7.5 and 7.8, however, the number of allocated – higher – 3.33 ± 0.27 (19.1%). The introduction of 18.54 ± 1.29 spores/grams of soil (3.67% of humus content) amounting to 18% of the deductions. Isolation Bac. anthracis with different pH indicates that the most spores of the pathogen are released within the pH range of 6.7–7.5. The content of humus in the soil affects the number of selected spores. With a higher content of humus, fewer amounts of spores is released anthracis, optimum pH for spore selection – 7.0.