Abstract
Three sialosphingolipids have been isolated from a lipid extract of gonads of the see urchin Echinocardium cordatum by partition dialysis and DEAE-cellulose column chromatography. The structure of the sialosphingolipid containing sulfate group has been established. On the basis of the results of total and partial acid hydrolysis, methanolysis, methylation, periodate oxidation and enzymatic hydrolysis with neuraminidase the sulfated sialosphingolipid was identified as 8-sulfate-sialyl-α-(2 → 6)glucopyranosyl-(1 → 1)ceramide. The longchain bases were mainly phytosphingosine and its C 16 homologue. The fatty acids of the sialosphingolipid were the mixture of normal and α-hydroxy fatty acids, their compositions were analysed by gas-liquid chromatography.
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