Abstract

Urease is an enzyme made by ureolytic microorganisms that hydrolyzes urea into ammonia and carbon dioxide. Microbial urease has wide applications in biotechnology, agriculture, construction, and geotechnical engineering. Urease-producing microbes can be isolated from different ecosystems such as soil, oceans, and various geological formations. The aim of this study was to isolate and characterize urease-producing bacteria from different natural sources. Ten urease-producing bacterial isolates were screened and selected using a qualitative urease activity assay. Among these, four isolates showed rapid urease activity. The isolates were further characterized with respect to their morphological, characteristics

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