Isolation and Screening of Microorganisms from Municipal Solid Waste for Production of Amylase

Abstract

One of the major problems of the present world is the management of municipal solid waste which in produced in enormous amount. For sustainable management of the solid waste, the use of microbial enzymes can be a better alternative over chemical conventional methods. Amylase is of great importance in textile, food and pharmaceutical industry. It can be used for biotransformation of wastes. The municipal solid waste can be used to isolate amylase producing bacteria that can help in bio-transformation of irritant municipal waste. Here, we have isolated two potent amylase producing strains from municipal solid waste, OW1 and OW2. These isolates were studied for their morphological, cultural and biochemical characters. The isolates were screened for the production of enzyme amylase. Optimization of growth of the isolates with respect to pH and temperature. The potential of the isolates for waste degradation was checked by weight loss method. The isolates were then tentatively identified as Pseudomonas aeruginosa and Bacillus subtilis.