Isolation and Screening of Fungal Isolates for Multienzyme Production Through Submerged and Solid State Fermentations

Vijay Kumar Garlapati

doi:10.4172/2155-9821.1000249

Abstract

Phytases, pectinases, and invertases are three major industrially important enzymes that find significant applications in food, feed, pharmaceutical and environmental sectors. The six isolated fungal species (JUIT, 1-6), isolated from soil Waknaghat, HP, have been identified as Aspergillus sp. through microscopical studies and fruiting body formation. Identified fungal isolates have been screened for multienzymes (Invertase, Pectinase and Phytase) production through solid state and Submerged Fermentations. Among all the fungal strains screened, Aspergillus nidulans (JUIT 4) and Aspergillus niger (JUIT 5) showed the highest coproduction of multi enzymes through Solid State Fermentation using wheat bran and maltose as a substrate and inducer, respectively. The Solid State Fermentation conditions seems to be equal to 2 ml spore suspension, 90% relative humidity, 0.4% water activity and 48 h incubation time. Partial purified (through 70% ammonium sulphate precipitation) enzymes have been further characterized for effect of pH. The resultant multienzymes complex with phytase, pectinase, and invertase activities may have considerable industrial potential in food and feed industries for fruit juice clarification, phytate elimination and in condiment preparations.

Highlights

Functional integration and the cooperative effect of multienzymes over individual counterparts and have demonstrated its important role in the production of different commodities in food and feed industries
Pectinase (EC 3.1.1.11) represents for pectic enzymes in brewing have been used in fruit juice extraction and clarification [3]. β-fructofuranosidase namely invertase (EC 3.2.1.26) is an predominant enzyme used in food industry for catalyzing the hydrolysis of terminal nonreducing β-fructofuranoside residues of β-fructofuranosides where fructose is preferred over sucrose due to its sweetness [4]
Six fungal isolates were isolated from agricultural fields of Delhi, Chandigarh and Himachal Pradesh of which four showed the coproduction of phytase, pectinase and invertase

Summary

Introduction

Functional integration and the cooperative effect of multienzymes over individual counterparts and have demonstrated its important role in the production of different commodities in food and feed industries. Agro-waste substrate utilization in SmF is an economical process for production of industrial enzymes such as Invertase, Pectinase and Phytase [17]. Production of multienzymes (pectinase, pectate lyase, cellulose, xylanase, β-xylosidase and invertase) through SSF have been reported in several instances through utilization Aspergillus, Fusarium, Neurospora and Penicillium sp using orange peel as a substrate [18]. In another study by Delabona et al [19] multienzyme preparations containing pectinase, cellulase and xylanase enzymes have been produced using six fungal isolates. Among the six fungal isolates tested, Aspergillus niger proved to be the most potent and produced highly active multienzyme systems. J Bioprocess Biotech 5: 249 doi:10.4172/2155-9821.1000249 isolates with their ability to produce multienzymes (phytase, pectinase and invertase) through SSF and SmF technologies using wheat bran as substrate

Materials and Methods

Results and Discussion

Conclusion