Abstract
The present study was conducted to explore the potential fungal isolates for maximum production of phytase by optimizing solid substrate preference and growth parameters (pH, temperature, fermentation time) of solid state fermentation (SSF). It has been inferred from the results that all the tested fungi (Aspergillus niger, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Cladosporium cladosporioides, Trichoderma reesei, and Trichoderma viride) showed inducible expression of phytase. Fungal isolates showed different preference of solid substrate out of wheat bran, lentil, oat, corn and bagasse for maximum production of phytase. Lentil and bagasse were not used preferably by any tested fungi. Among the tested fungi, maximum phytase production was observed in A. flavus (80 U/g of solid substrate) using wheat bran as solid substrate at pH 6 after 7 days of fermentation period at 30°C. It was established that solid substrates with high phytate and low inorganic phosphate (Pi) contents are substrate of choice for phytase production by SSF. Key words: Phytase, solid state fermentation, optimization.
Highlights
Cereals, legumes and oilseed crops are cultivated globally over approximately 90% of agricultural land and serve as major source of nutrients for animal kingdom (Reddy et al, 1982)
Maximum phytase production was observed in A. flavus (80 U/g of solid substrate) using wheat bran as solid substrate at pH 6 after 7 days of fermentation period at 30°C
The selected fungi were assessed for phytase production under induced and uninduced conditions
Summary
Optimization of phytase production in solid state fermentation by different fungi Iram Gull, Amber Hameed, Muhammad Shahbaz Aslam and Muhammad Amin Athar. The present study was conducted to explore the potential fungal isolates for maximum production of phytase by optimizing solid substrate preference and growth parameters (pH, temperature, fermentation time) of solid state fermentation (SSF). It has been inferred from the results that all the tested fungi (Aspergillus niger, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Cladosporium cladosporioides, Trichoderma reesei, and Trichoderma viride) showed inducible expression of phytase. Fungal isolates showed different preference of solid substrate out of wheat bran, lentil, oat, corn and bagasse for maximum production of phytase.
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