Isolation and Screening of Bioactivities from Bacillus sp. Isolated from Banana Rhizosphere

Abstract

Bacillus sp. has been widely known to produce various bioactive metabolites related to plant growth. Bacillus sp. has also been known as one of the plant growth-promoting bacteria. In this study, a total of 68 isolates of Bacillus sp. were isolated from the rhizosphere of banana plantation using soil suspension technique. Isolated Bacillus sp. was then subjected to screening of their secondary metabolites such as cellulase, mannanase, xylanase, indole-acetic-acid, and gibberellic acid production before potential isolates were chosen and identified using their 16S rRNA and later subjected to seed germination testing. It was observed that Bacillus sp. isolated produces most of cellulase but did not have much affinity to produce gibberellic acid as only 5 Bacillus sp. were observed producing gibberellic acid. Three of the potential Bacillus sp. were selected and identified as Bacillus pumilus strain PNO21, Bacillus amyloliquefaciens strain PNO7 and Bacillus wiedmannii strain PNO28. Seed germination test also revealed that these 3 Bacillus sp. did not inhibit the seeds germination.