Abstract
BackgroundThis study is aimed to evaluate the use of epithelial cells adhering the acrylic removable partial denture in establishing the sex of the individual using sex-determining region of the Y chromosome (SRY) gene. With a hypothesis that prosthetic appliance can also be used as a source of DNA in forensics for sex determination. A sample of 30 partial dentures from different denture wearers was selected for this study. Informed consent was taken from the subjects and institutional ethical clearance was obtained for this study. Samples were then washed in saline and scrapings from 0.5 × 0.5 cm2 of the tissue contacting surface were taken using a blade of fixed thickness. DNA extraction was done from these isolated epithelial cells using Real Genomics YGB 100 (Real Biotech Corporation, Taiwan). Quantification and purity assessment of the extracted DNA was done using a Nanodrop ND-1000 spectrophotometer and clean DNA was subjected to real-time PCR amplification using SRY sex-typing marker for the detection of the presence of the SRY gene.ResultsIsolation of epithelial cells and extraction of DNA from all 30 acrylic removable partial dentures were possible. Average DNA yield quantified from all samples was 32.37 ng/μl with a minimum yield of 2.17 ng/μl and a maximum yield of 117.57 ng/μl. On amplification of the target sequence, sex determination was possible from all samples with 100% accuracy.ConclusionAcrylic removable partial denture can also be used as a source of DNA in forensics for sex identification.
Highlights
This study is aimed to evaluate the use of epithelial cells adhering the acrylic removable partial denture in establishing the sex of the individual using sex-determining region of the Y chromosome (SRY) gene
A total number of 30 removable partial dentures made of polymethyl methacrylate was collected from 30 individuals which included equal distribution of male and female
The age, sex and missing teeth of the individual which were replaced by the removable acrylic partial denture and their corresponding Deoxyribonucleic acid (DNA) yield are tabulated (Table 1)
Summary
This study is aimed to evaluate the use of epithelial cells adhering the acrylic removable partial denture in establishing the sex of the individual using sex-determining region of the Y chromosome (SRY) gene. Identification of an individual, living or dead, is based on the theory that all individuals are unique. These distinct characteristics aid in identifying and distinguishing an individual. Various methods are employed by forensic odontologist in establishing the sex using teeth, skull and other oral tissues, of which most routinely used methods are visual or clinical method, microscopic method and advanced methods (Byers et al 1997; Işcan and Sema Kedici 2003; Bharath 2011; Steyn and Işcan 1998; Franklin et al 2006). Advanced methods in sex determination involve isolation of DNA from the biological samples and subjecting them to various sex-typing markers to obtain accurate
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