Isolation and process parameter optimization ofBrevibacterium caseifor simultaneous bioremediation of hexavalent chromium and pentachlorophenol

Abstract

Chromate and pentachlorophenol are major pollutants discharged through tanneries. Three bacteria resistant to high Cr(6+) and PCP concentrations simultaneously were isolated. The TVS-3 strain was tolerant to highest 850 mg l(-1) Cr(6+) and 1000 mg l(-1) PCP concentration and concomitantly reduced 69% Cr(6+) and degraded 72% PCP within 168 h at pH 7.5, 35 ± 1°C temperature, was selected and identified as Brevibacterium casei. At 168 h of growth, bacterium showed maximum PCP utilization of 720 mg l(-1) and released 900 mg l(-1) chloride ion. The bacterium exhibited remarkable ability to significantly reduce Cr(6+) and degrade PCP in presence of other metals, between 100-120 rpm aeration and over broad pH (6.5-10.0) and temperature (30-40°C) range. Maximum 78% Cr(6+) reduction and 82% PCP degradation was observed at pH 8.0, 35 ± 1°C within 168 h of incubation, 120 rpm and initial concentration of 850 mg l(-1) Cr(6+) and 1000 mg l(-1) PCP. This is the first study reporting 78% Cr(6+) reduction and 82% PCP degradation simultaneously by single native bacteria under wide growth conditions utilizing PCP as sole carbon source. This bacterium may potentially be useful for simultaneous bioremediation of Cr(6+) and PCP containing wastes in the environment.