Abstract
The hydrogen-evolving bacterium Clostridium butyricum IFO 3847 was found to carry three plasmids: pSSK1, pSSK2, and pSSK3. The molecular weights of pSSK1, 2 and 3, determined by agarose gel electrophoresis and electron microscopy, were about 51, 32, and 9.4 megadaltons respectively. The two larger plasmids were analysed by digestion with several restriction enzymes such as AvaII, BamHl, EcoRl, Pst1, and Sall. With each enzyme, 10–20 fragments were produced from pSSK1 and five to six fragments from pSSK2. Since in each digestion some fragments were common to both plasmids, the two plasmids pSSK1 and pSSK2 must be related to each other to a high degree. The other small plasmid pSSK3 was digested by restriction endonucleases, EcoR1, Pst1, and Sall at single sites, so that this plasmid might be a candidate as a vector for gene cloning in Clostridium species.
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