Abstract
This chapter discusses rapid alkaline extraction method for the isolation of plasmid DNA. Plasmids are double-stranded circular DNA molecules that have the property of self-replication, independent of chromosomal DNA. Isolation of plasmid DNA requires that it be separated from host-cell chromosomal DNA as well as other macromolecular components. Screening procedure has been used successfully with other E. coli strains, with other bacteria, and with large plasmids. Plasmid DNA in extracts can exist in a number of different forms that can make bands on a gel sometimes difficult to identify unambiguously. An important next step in the characterization of plasmids is often an examination of the fragments produced by digestion with different restriction enzymes.
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