Abstract
In recent years, the emergence of avian orthoreovirus (ARV) has caused significant losses to the poultry industry worldwide. In this study, a novel ARV isolate, designated as AHZJ19, was isolated and identified from domestic chicken with viral arthritis syndrome in China. AHZJ19 can cause typical syncytial cytopathic effect in the chicken hepatocellular carcinoma cell line, LMH. High-throughput sequencing using Illumina technology revealed that the genome size of AHZJ19 is about 23,230 bp, which codes 12 major proteins. Phylogenetic tree analysis found that AHZJ19 was possibly originated from a recombination among Hungarian strains, North American strains, and Chinese strains based on the sequences of the 12 proteins. Notably, the σC protein of AHZJ19 shared only about 50% homology with that of the vaccine strains S1133 and 1733, which also significantly differed from other reported Chinese ARV strains. The isolation and molecular characteristics of AHZJ19 provided novel insights into the molecular epidemiology of ARV and laid the foundation for developing efficient strategies for control of ARV in China.
Highlights
Avian orthoreovirus (ARV) belongs to the Orthoreovirus genus (Reoviridae family) with a double-stranded and segmented RNA genome [1, 2]
Sequence analysis revealed that AHZJ19 was significantly different from the avian orthoreovirus (ARV) previously isolated in China, and is a novel recombinant ARV originated from Hungarian strains, North American strains, and Chinese strains
The mortality caused by ARV is not high, the infection of ARV generally results in immunosuppression, affects the normal growth and production performance of broilers, and increases the susceptibility to other pathogens
Summary
Avian orthoreovirus (ARV) belongs to the Orthoreovirus genus (Reoviridae family) with a double-stranded and segmented RNA genome [1, 2]. A novel ARV strain, designated as AHZJ19, was isolated from a chicken flock with viral arthritis syndrome using LMH cells, and its complete genome sequence was determined. Sequence analysis revealed that AHZJ19 was significantly different from the ARVs previously isolated in China, and is a novel recombinant ARV originated from Hungarian strains, North American strains, and Chinese strains.
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