Isolation and mapping of Mu nu mutants which grow in him mutants of E. coli

Abstract

Mu nuA and Mu nuB mutants were selected by their ability to form plaques on lawns of Escherichia coli himA and himB mutants, respectively. Deletion mapping of the nuA and nuB mutations by marker rescue from λpMu transducing phages or from hosts containing deleted Mu prophages indicated that nuA mutations are located within or to the left of gene A and that nuB mutations are located within or between Mu genes G and I.