Correction and refinement of the genetic map of bacteriophage Mu

Abstract

Three hundred sixty-one amber mutations in essential genes of bacteriophage Mu have been assigned to 1 of 76 deletion groups on the basis of results of deletion mapping by marker rescue from 90 λpMu transducing phage. The original order of previously defined cistrons determined from prophage deletion mapping has been confirmed with the exception that cistron O has been deleted and cistron H has been found to map between cistrons E and F rather than between cistrons G and I. Mapping of mutations in the new cistrons T, U, V, W. and Y ( Howe et al., 1979a, Virology 93, 303–319 ) has produced the following cistron order: ABClysDEHFGITJKLMYNPQVWRSU.