Abstract
WHILE germination of conidia of the obligate parasite Peronospora tabacina Adam. has been effected on tobacco leaf disks1,2, it has not previously been possible to complete the life cycle of the fungus on such disks, because of their rapid deterioration. Sporulation investigations have been possible only by taking disks from leaves which had been infected previously and in which mycelium was well established3. Izard4 has described a method for the growth of P. tabacina on leaves and leaf disks in the laboratory, but in our experience this method has been unsuccessful with the Australian varieties of host and pathogen. Accordingly, we have devised the following technique.
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