Abstract
Using inocula from a variety of sources, mixed cultures of methanol-utilizing Bacillus strains were enriched readily at 55°C. Isolation of pure cultures was difficult; the six strains that were obtained eventually in pure culture all possessed the RuMP pathway, grew rapidly on methanol at temperatures up to 60° C, and were tolerant to very high methanol concentrations. An NAD-dependent alcohol dehydrogenase appeared to be involved in the initial oxidation of methanol.
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