Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea.

Chengyuan Ji,Huochun Yao,Ruini Sun,Jiale Ma,Zihao Pan,Yao Zhang

doi:10.3390/v13112217

Abstract

Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus.

Highlights

Bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle
From the latest classification of enteroviruses, bovine enterovirus (BEV) is classified as EV-E and EV-F
We report the isolation and characterisation of an EV-F1 strain of BEV from a herd of cattle in Hebei Province that had severe symptoms of diarrhoea

Summary

Introduction

Bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. We designated this strain as HB19-1 in this study. The enterovirus genus within the picornaviridae includes nine enteroviruses (A–J) and three rhinoviruses (A–C) [1] These viruses share similar characteristics and are the primary pathogens associated with respiratory and gastrointestinal diseases amongst humans and animals. Previous studies have suggested that it is only of limited pathogenicity, as no clinically significant signs of BEV infection have been

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