Isolation and identification of the most important aflatoxin B1-producing species in Najaf Governorate respiratory patients

Abstract

The purpose of this study was to evaluate the efficacy of some biological methods for removing toxins or reducing the toxic effect of aflatoxin. Initially, the genus Aspergillus spp was isolated from chronic lung infections, Sputum samples were collected from patients with chest and respiratory diseases who visited the consulting clinic, and the samples were subjected to direct microscopic examination and agricultural and molecular diagnosis using PCR technology. Aflatoxin B1 chemical detection also indicated the use of some poison detection methods, such as the ammonia vapour method, a kind-layer chromatic analysis, and high performance chromatography. The tested isolation gave the same result as the ammonia test for ultraviolet rays by changing the color of the inverted dish to red-carbon and expressing one isolation as a negative result of the test.