Isolation and Identification of Tersakan Stream (Amasya, Suluova TÜRKİYE) Cyanobacteria and Investigation of the Presence of the Microcystin-LR

Abstract

Cyanobacteria are a concern worldwide due to their blooms and the toxins they produce. The aim of this study is to reveal the cyanobacterial diversity of Tersakan Stream and toxin analysis. For these purpose, water samples were taken from Tersakan Stream in 2018 and cyanobacterial diversity was determined by culture-dependent and independent methods. PCR amplification of microcystin genes (McyB and McyE) and Microcystin-LR (L:Leucine, R: Arginine) (MC-LR) analysis in water sample was performed. While 10 different Cyanobacteria were identified by light microscopy examination and 16S rRNA + ITS sequence, less diversity was revealed by culture-independent methods. Sequences of the isolates were associated with Pseudanabaena lonchoides, Leptolyngbya sp, Toxifilum sp, Cylindrospermum moravicum, Nostoc sp, Oscillatoria sp, Synechocystis sp, Synechococcus sp, Cyanobacterium aponinum and Cyanobium sp. Culture-independent sequences were associated with Microcytis sp, Nostoc sp, Cyanobium sp, Dolichospermum sp, Anabaena sp, Leptolyngbya sp and Uncultured cyanobacterium. Microcystin analysis was performed by both molecular and analytical methods. PCR results of McyB and McyE genes supported the HPLC-DAD analysis result. 0.467 μg L-1 microcystin LR was detected in Tersakan Stream by HPLC-DAD analysis. Although the microcystin concentration is low, Tersakan Stream is an important area to be monitored with its eutrophic character and cyanobacterial diversity.