Isolation and Identification of Some Genes for Drought Tolerance in Suaeda Sp Plant

Rasha Khalil,El-Shaimaa El-Demerdash

doi:10.21608/eajbsc.2019.61509

Abstract

In the present study, two species of the drought-tolerant genus Suaeda (Suaeda vera and Suaeda pruinosa) were investigated for detection of some genes responsible for drought resistance. Three genes (P5CS, BADH, and DREB) were chosen and detected using specific primers producing bands of different sizes 1500bp, 700bp and 430bp, respectively. The obtained fragments of the three genes were Sequenced, and phylogenetic tree constructed. The results revealed the efficiency of BADH to clarify the closest relatedness of Suaeda species with other species on National Center for Biotechnology Information (NCBI) database. In addition, one can conclude that BADH gene may be included in drought resistance mechanism of Suaeda species. This study can be used in the future for breeding and crop improvement programs..

Highlights

Environmental stress, such as low temperature, high temperature, high salinity, and drought restrict the distribution and productivity of plants
Proline is synthesized from glutamate or arginine /ornithineIn higher plants, P5CS is encoded by a nuclear gene from Vigna aconitifolia (Hu et al, 1992), Arabidopsis thaliana (Strizhov et al, 1997), Glycine max and Lactuca sativa (Porcel et al, 2004) and other species
Homology search results in National Center for Biotechnology Information (NCBI) showed that Dehydration- responsive element-binding proteins (DREBs) gene had high identity to other plants such as Glycine max (96% identities, accession number KT031079.1), Vigna radiata (94% identities, accession number XM 01464.1), Lupinus albus (94% identities, accession number CP023115.1), Phaselous vulgaris (92% identities, accession number FQ393912.1), Cajans cajan (90% identities, accession number XM020383974.1) Carica papaya (90% identities, accession number KU O65116.1) Rosa drinensis (89% identities, accession number XM024308992.1)

Summary

INTRODUCTION

Environmental stress, such as low temperature, high temperature, high salinity, and drought restrict the distribution and productivity of plants. When subject to salt stress or drought, some vascular plants typically respond with increased accumulation of proline and glycine betaine, an important osmoprotectant that is produced in response to salt and other osmotic stresses Zhou et al, (2008). Detected an increase of glycine betaine and proline in Suaeda fruticosa and S. vera under salinity and drought stress. Proline is synthesized from glutamate or arginine /ornithineIn higher plants, P5CS is encoded by a nuclear gene from Vigna aconitifolia (Hu et al, 1992), Arabidopsis thaliana (Strizhov et al, 1997), Glycine max and Lactuca sativa (Porcel et al, 2004) and other species. Three identified drought-responsive genes were detected in two Suaeda species in order to clarify their role in plantresistance

MATERIALS AND METHODS

Findings

CONCLUSION