Isolation and Identification of Seaweed-Associated Bacteria and Their Antibacterial Activity against Skin Disease Agents

Abstract

Bacteria associated with marine organisms have become the focus of worldwide research for their potential to generate bioactive substances, such as antibacterial compounds. This study aimed to identify the antibacterial activity of seaweed-associated bacteria in Teluk Awur Jepara, Indonesia, against Propionibacterium acnes and Staphylococcus epidermidis. Three seaweed species, Caulerpa racemosa, Padina minor, and Halimeda opuntia, were sampled in this study. Their isolates were selected tested for antibacterial activity against pathogenic bacteria using the paper disc diffusion and Minimum Inhibitory Concentration methods and were molecularly identified based on the 16s RNA gene (27F-1492R). Twenty-one isolates were isolated from the 3 seaweed species: 11 from C. racemosa, 6 from P. minor, and 4 from H. opuntia. Further testing revealed potential isolates (C2a, C2c, C2d, and H2d) with antibacterial activity against P. acnes F2 ATCC 6919 and S. epidermidis FNCC-0048. Gene-based identification using 16s RNA (27F-1492R) demonstrated the occurrence of 4 bacterial species, namely Vibrionaceae bacterium PH25 (99.86 %), Vibrio alginolyticus strain GS MYPK1 (99.65 %), Salinivibrio costicola strain M318 (99.86 %), and V. alginolyticus strain 2014V-1011 (99.93 %). HIGHLIGHTS Bacteria associated with racemosa, P. minor, and H. opuntia can show strong antibacterial properties capable of mitigating the growth of P. acnes F2 ATCC 6919 and S. epidermidis FNCC-0048 Seaweed-associated bacteria and their antibacterial activity against skin disease agents. Gene-based identification using 16s RNA (27F-1492R) demonstrated the occurrence of 4 bacterial species, namely Vibrionaceae bacterium PH25 (99.86 %), Vibrio alginolyticus strain GS MYPK1 (99.65 %), Salinivibrio costicola strain M318 (99.86 %), and alginolyticus strain 2014V-1011 (99.93 %) The major bacteria species on the scalps of those suffering from dandruff, we believe these isolates may be useful for dandruff therapy GRAPHICAL ABSTRACT