Active Compound Phenyl Ether from Potential Endophytic Fungi of Syzygium zeylanicum

Abstract

Endophytic fungi can be found in various types of plants, especially medicinal plants, such as Syzygium zeylanicum, one of the many medicinal plants found in Indonesia. This study aimed to obtain isolates of endophytic fungi from the bark of Syzygium zeylanicum, L., with antibacterial and antioxidant activities and identify their bioactive compounds. Characterization of endophytic fungi was carried out morphologically and fungi that showed high bioactivity were characterized molecularly. Antibacterial activity was carried out by disc diffusion method and antioxidant activity by DPPH method. Isolation of bioactive compounds was carried out by chromatographic techniques and determination of the structure of pure chemical compounds by spectroscopic analysis. In this study, 3 isolates of endophytic fungi were obtained. Based on their morphological characterization, the endophytic fungi belong to the genera Trichoderma (Code SZT2 and SZT6), and Sclerotium (Code SZT8). Trichoderma mushroom extract (SZT2) gave high antibacterial activity (74.0 % against E. coli; 78.2 % against S. aureus; 80.5 % against S. tyhpi and 74.0 % against B. subtilis). The antioxidant activity was very strong SZT2 (IC50 = 5.83 µg/mL) SZT2 potential endophytic fungus based on molecular analysis is Trichoderma koningi. SZT2 mushroom extract has an active compound in the form of compound 3-hydroxy-4(hydroxy(4-hydroxyphenyl)methyl)-ɣ-butyrolactone based on the results of compound isolation using NMR and HMR. These endophytic fungal compounds can be developed into new sources of antibacterial and antioxidant bioactive compounds through a series of further studies. HIGHLIGHTS New movement in the discovery of antioxidant and antibacterial compounds with endophytic fungal sources. in this study we found compound A which have very strong antioxidant activity accompanied by strong antibacterial activity against 4 pathogenic bacteria. GRAPHICAL ABSTRACT