Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance

Abstract

The bacteria Pseudomonas aeruginosa were isolated and diagnosed and compared with Pseudomonas fluorescens for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and Pseudomonas fluorescens and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitative level using the phytic technique. Carry out a sensitivity test for antibiotics, depending on the method of dissemination of the disc (Kypri Power). As (25 bacterial isolates were isolated from Pseudomonas aeruginosa, with a percentage of (21.7%)., and (10) bacterial isolates from Pseudomonas fluorescens, with a percentage (8.7%). As for P. putida and P.stutz, the total number of isolates for each of them was (3). Isolates with a percentage of (2.7%).The infection of males with these bacteria was higher than that of females out of a total of 41 isolates of interest to our study, as it appeared that there were (24) infected males, i.e. (58.5%), and (17) infected females, representing a percentage of (41.5%). The sensitivity of these isolates was tested against a group of antibiotics, including 7 antibiotics. The results of the current study showed that the Pseudomonas aeruginosa isolates were highly resistant to Piperacillin, Tetracycline, and Gentamicin, where it formed a high resistance rate of 100%, while the study isolates showed sensitivity to Tobramycin antibiotics. Ciprofloxaci by (90%). As for Pseudomonas fluorescens, the percentage of resistance to Co-trimoxazole was 80%, Tetracycline 50%, tobramycin 40%, cefepime 60%, Ciprofloxacin 60%, Piperacillin 40%, Gentamicin 70%.