Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia.

Wisiva Tofriska Paramaiswari,Dodi Safari,Wahyu Finasari Said,Miftahuddin Majid Khoeri,Nurma Sumar Sidik,Wisnu Tafroji,Joseph Falkinham

doi:10.1155/2021/6646925

Abstract

Aim To investigate optochin-resistant viridans group streptococci (VGS) strains isolated from the sputum sample of adult patients with different clinical symptoms. Materials and Methods Optochin-resistant VGS isolates were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). recA sequencing was used to confirm identified isolates at the genus level by MALDI-TOF MS. Finding. We identified 79% of tested isolates (148/187) at the species-level identification using the MALDI-TOF MS tool. We identified that the most common species isolated from sputum specimens were S. oralis (44.9%) followed by S. mitis (25.7%), S. infantis (9.1%), S. parasanguinis (7.5%), S. peroris (3.7%), S. anginosus (2.7%), and S. sanguinis (2.1%). Discussion. The S. oralis strains were majority of optochin-resistant VGS isolates obtained from sputum of adult patients in Jakarta, Indonesia. MALDI-TOF MS showed potential for the rapid identification tool to identify optochin-resistant VGS isolates. Although there were discrepancies in identifying isolates at the genus/species level, the performance could be improved by expanding its database.

Highlights

Wisiva Tofriska Paramaiswari,1 Nurma Sumar Sidik,2 Miftahuddin Majid Khoeri,1 Wisnu Tafroji,1 Wahyu Finasari Said,3 and Dodi Safari 1
MALDI-TOF MS identified 79% (148/187) isolates with score value ranging from ≥2.000–3.000, indicating the highly probable species identification result. e majority identified species was S. oralis (50.7%), followed by S. mitis (31.1%), S. parasanguinis (9.5%), S. anginosus (3.4%), S. sanguinis (2.7%), S. peroris (2.0%), and S. pseudopneumoniae (0.7%) (Table 1)
We observed that 21% (39/187) of optochin-resistant viridans group streptococci (VGS) isolates were identified at the genus level (ID score value: 1.700–1.999) with majority isolates identified as S. oralis (38.5%) followed by S. mitis (23.1%), S. peroris (20.5%), S. pneumoniae (12.8%), S. parasanguinis (2.6%), and S. infantis (2.6%) (Table 2). e identification scores obtained from the isolates extracted using the standard

Summary

Research Article

Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia. Aim. To investigate optochin-resistant viridans group streptococci (VGS) strains isolated from the sputum sample of adult patients with different clinical symptoms. E S. oralis strains were majority of optochin-resistant VGS isolates obtained from sputum of adult patients in Jakarta, Indonesia. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has become an indispensable tool for clinical microbiology laboratories and shown to be a potential alternative for organism identification with a rapid and cost-saving method for VGS identification [2, 5, 9]. We investigate further nonpneumococcus strains from adult patients for optochin-resistant VGS identification by the MALDI-TOF MS

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