Application value of MALDI-TOF-MS in proteomics of hepatitis B virus-related liver cancer

Abstract

Objective To explore the application value of matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF-MS) inproteomics of HBV-related liver cancer. Methods Thirty patients with HBV-related liver cancer, and 25 healthy volunteers were selected from January2018 to June 2018 in our hospital. The serum proteins of these participantswere captured by weak cation exchange (WCX) beads. MALDI-TOF-MS and related software were used to analyze the proteins, detect the marker of serum proteins in HBV-related liver cancer, establish the liver cancer diagnosis model and perform blind test. Results Totally 81 protein peaks were screened out, and 27 protein peaks had significant difference (P<0.05), of which 17 were up-regulated, 10 were down-regulated. Three differential protein peaks (M/Z 9179.55, 7789.00, 4097.00) were screened by BPS5.0 software to establish the model. Further blind test showed that the sensitivityand specificity of the protein peaks were 90.91% and 77.78%, respectively. Conclusions MALDI-TOF-MS provided an important basis for the diagnosis and treatment of HBV-related liver cancer. Key words: MALDI-TOF-MS; Hepatctis B virus; Liver cancer; Proteomics