Abstract
A moderately halophilic bacteria strain with cellulase (CMCase) activity, ZSCW-10, which can survive under wide range of NaCl concentration (1 %~15 %, w/v) and pH (4.5~9.0), was isolated from the sediment of intertidal zone located in the Xiangshan Island, Zhejiang Province. The strain was identified based on 16S rDNA sequence determination and phylogenetic analysis together with its morphological and physiological characterization. The results indicated that the 16S rDNA homologies were 97% between strain ZSCW-10 and some strains of Halomonas sp.. The phylogenetic tree was constructed with Molecular Evolutionary Genetics Analysis version 5 (MEGA5), showed the closest relationship between strain ZSCW-10 and Halomonas venusta. Based on its physiological and biochemical properties, homology and phylogenetic analysis, strain ZSCW-10 was identified as a subspecies of Halomonas sp.. The optimum culture conditions were: NaCl 6.0%, 32°C, pH 7.5.
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