Isolation and Identification of High Efficient Oil-Degrading Bacteria and Formulation of Complex Bacteria

Abstract

In this experiment, 25 oil degrading bacteria strains were isolated from the beach near Dalian Bay. The top 4 high efficient oil-degrading bacteria（SQ、SY 、SW、SC） were selected to be studied. Using Physiological and biochemical identification, electron microscopy, and 16S rDNA sequence analysis, strain SQ was identified as Advenella kashmirensis，SY was identified as Achromobacter xylosoxidans, SW was identified as Acinetobacter venetianus，SC was identified as Alcaligenes xylosoxidans subsp.xylosoxidans. For the first time this experiment provided reference to application and degradation characteristics of Advenella kashmirensis in crude oil treatment. These four marine oil degrading bacteria were selected to form bacterial consortium. The most efficient mixed strains were constructed by using these four strains with proportional combination. The oil removal ratio of the optimal mixed strains increased to 70.33%, nearly 25% higher than the highest degradation rate of single bacterium, showing significant synergism in the bacterial consortium. Advenella kashmirensis enhanced oil degradation obviously, playing a crucial role in the bacterial consortium.