Isolation and identification of compounds from Phaleria macrocarpa (Scheff.) Boerl fruit extract

Abstract

ObjectiveTo identify and isolate the chemical compounds of Phaleria macrocarpa (P. macrocarpa) fruit ethanolic extract. MethodsDried fruit of P. macrocarpa was extracted with 90% ethanol and partitioned between n-hexane/H2O and ethyl acetate/H2O. The organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance (NMR), carbon-13 NMR)], 2D-NMR (heteronuclear multiple-quantum correlation and heteronuclear multiple-bond correlation), and mass spectrum. ResultsPurification of n-hexane and ethyl acetate fractions from ethanolic extract of P. macrocarpa fruit resulted in isolation of nine compounds. ConclusionsA new compound was isolated and identified as glyceryl pentacosanoate. Also, two xanthones, which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are firstly reported to be isolated from P. macrocarpa.