Abstract
Tofu liquid waste contains high levels of organic matter, especially protein and amino acids. These organic compounds can be proteins, carbohydrates and fats. Most of the bacteria are able to use oil or fat as a source of carbon and energy, bacteria that have this ability are often known as lipolytic bacteria. This study aims to obtain biosurfactant producing bacteria from tofu wastewater. This research was conducted from July - September 2020. The method used in this research is survey method and emulsification method. The media used for bacterial isolation were Tryptic Soy Broth (TSB) and Tryptic Soy Agar (TSA). 6 of the isolates are able to produce biosurfactants. Morphological and biochemical characteristics indicate six bacterial genera, namely Genus Agrobacterium, Proteus, Proteus, Citrobacter, Enterobacter, and Serratia. The bacteria obtained are bacteria that have the potential to degrade oil in a polluted environment.
Highlights
This study aims to obtain biosurfactant producing bacteria from tofu wastewater
Plant Pathogenic Fungus Rhizoctonia solani in Response to the AntagonisticBacteria Serratiaproteamaculans and Serratia plymuthica. BMC Genomics. 16: 630. Limbah Tahu terhadap Pertumbuhan dan Hasil Tanaman Kacang Hijau (Vigna radiata L). Jurnal Agrotropika Hayati, 3. Hidayat, N., C. Masdiana, dan S. Suhartini. (2006). Mikrobiologi Industri. ANDI. Yogyakarta
Summary
Hasil pengujian emulsifikasi Gambar 1. bakteri yang berasal dari limbah cair tahu UD. Isolat bakteri yang memiliki aktivitas biosurfaktan tertinggi ialah TH D2 dengan indeks emulsi sebesar 64%. Aktivitas biosurfaktan terendeh ditunjukkan oleh isolat TH C1 dengan indeks emulsi sebesar 26% karena sebagian bakteri tidak mampu memanfaatkan minyak dan lemak sebagai sumber karbon dan energinya. Hal ini sesuai dengan pernyataan Okpokwasili et al (2006) bahwa biosurfaktan dapat disintetis dari bahan dasar organik yang melimpah yaitu karbohidrat, lemak dan protein. Faktror-faktor yang sangat berpengaruh terhadap produksi biosurfaktan adalah sumber karbon, sumber nitrogen, trace element dan faktor lingkungan seperti pH, suhu, agitasi, dan kertesediaan oksigen. Berdasarkan penelitian Purnomohadi (2011), bakteri yang menghasilkan biosurfaktan adalah memiliki indeks emulsifikasi berkisaran antara 30% sampai 80%. Identifikasi Bakteri Karakteristik morfologi koloni Bakteri dibedakan berdasarkan pengamatan dengan bentuk, warna, tepian, dan elevasi yang berbeda pada setiap bakteri. Bentuk koloni bakteri dengan bentuk, warna, tepian, dan elevasi dapat dilihat pada Tabel 1
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