Abstract
Berberis aristata (Berberidaceae) is an influential medicinal plant and found in the different provinces of the world. It has compelling medicinal value in the traditional Indian system of medicine. More multitudinous secondary metabolites are contemporary within the plants, which helps to cure the disease. The chromatographic techniques have significant role in natural products chemistry and discovery of innovative compounds of pharmaceutical and biomedical importance. The aim of the present study is to evaluate sequestration of secondary metabolites using column-chromatographic techniques and this metabolites can be further secluded, identified by using LC-ESI-Q-TOF-MS system and also performed its antimicrobial activity. These metabolites were characterized for determination of medicinal properties. This study helps to identify the presence of secondary metabolic compounds in the plant.
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