Abstract
<p>Chromatographic techniques have significant role in natural products chemistry as well as contribute dramatically in the discovery of novel and innovative compounds of pharmaceutical and biomedical importance. This study focused on step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatographic involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions; and e) Analysis of each fractions using thin layer chromatography. However, depending on nature of research, compounds can be further purified using high performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectral analyses.</p><p><strong>Video Clips</strong></p><p><a href="https://www.youtube.com/v/pr8mrBoI8xA">Part 1:</a> 3 min 45 sec</p><p><a href="https://www.youtube.com/v/rYrfClKn-og">Part 2:</a> 6 min 21 sec</p><p><a href="https://www.youtube.com/v/kffHXxuPwbo">Part 3</a>: 4 min 45 sec</p>
Highlights
Plants are healthy and natural resource of life (Russell and Duthie, 2011)
Medicinal plants are of great importance with endless therapeutic properties useful for curing various diseases with an advantage of being natural (El-Shemy et al, 2007)
There is an urgent need to search for alternative remedies as naturally occurring biologically active secondary metabolites from plant origin
Summary
Plants are healthy and natural resource of life (Russell and Duthie, 2011). In particular, medicinal plants are of great importance with endless therapeutic properties useful for curing various diseases with an advantage of being natural (El-Shemy et al, 2007). The plant or microbial extracts in crude or partially-purified forms were the only sources of medication available for the treatment of human and animal diseases. This gave an idea that the effect of a drug in human body is due to an interaction of drug with biological molecules. This opened new doors in pharmacology, as pure, isolated chemicals, instead of extracts, as the standard for the treatment of diseases. The objective of this study was to provide step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques
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